Equilibrium dialysis method, a classic method for determining plasma protein binding rate
Drugs are bound to plasma proteins to varying degrees in plasma, and the degree of binding can affect the drug's absorption, distribution, metabolism, and excretion in vivo, which in turn can affect the pharmacodynamic behavior of the drug. In general, after absorption into the bloodstream, only free drug reaches the site of action and produce pharmacological activity. Equilibrium dialysis is one of the most commonly used method to determine the free concentration of medicines based on the equilibrium principle of drug binding. It is the classical method to study drug plasma protein binding rate. It has been found that the critical physiological and pharmacological functions of proteins require the participation of small drug molecules, which can promote or inhibit the protein functions through their interaction with proteins. The binding of drugs to plasma proteins can affect the biological activity of drugs. Therefore, determining the drug plasma protein binding rate is m...