Analysis of the role of Biacore assay in drug development
SPR biosensors have a wide range of applications in life sciences, drug residues, food detection, disease mechanisms, etc. due to their advantages of no labeling, online detection, reproducibility, no sample pre-treatment, etc. In the fields of protein network regulation and cellular signal transduction, it is often necessary to search for the unknown factors interacting with a known protein to discover the whole regulatory network. Surface plasmon resonance (SPR) technology is a biosensing analysis technique for analyzing interactions between biomolecules and is now commonly used in the Biacore, a biomolecular interaction analysis system. The Biacore assay can be used for the analysis of binding specificity between biomolecules, concentration quantification, binding kinetics and affinity analysis, and thermodynamic analysis, enabling real-time detection of biomolecular interactions between DNA and proteins, between protein molecules, between drugs and proteins, betwee